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Status

v1.0 (March 26, 2008): This Sorghum bicolor assembly was built using the Arachne assembler with a data freeze from January 25, 2007. After the build, 28 breaks and 108 manual joins were made. 10 of these joins were across centromeres, and the size of the centromere was estimated for each chromosome based upon the amount of centromeric sequence already assembled for that chromosome. The main genome is in 10 chromosomes with many small unmapped pieces, some of which contain homologous rice genes. The Sorghum bicolor mitochondria and chloroplast were previously sequenced and are available in Genbank at NC_008360 and NC_008602. The nuclear genome sequence from the assembly was annotated using the JGI Genome Annotation Pipeline, collaborator-contributed gene models, and custom analyses.

Summary statistics for the S. bicolor v1.0 release:

Nuclear Genome Assembly
v1.0
Nuclear genome size (Mbp)
 739
Sequencing read coverage depth
8x
Reported # of contigs
3,376
# of nuclear scaffolds
3,304
# of nuclear scaffolds >2 Kbp
3,217
Nuclear scaffold N/L50
6/62 Mbp
Three largest Scaffolds (Mbp)
78
74
74

 

Gene Model Track
Sbi1_4
FilteredModels6
length (bp) of:
average
average
gene
2,856
2,794
transcript
1,426
1,236
exon
267
279
intron
419
456
description:

protein length (aa)
409
359
exons per gene
4.8
4.4
# of gene models
34,496
35,899

 

ESTs
Data set
# sequences total
# mapped to genome
# not mapped to genome
% mapped to genome
EST clusters
EstClusters_SBGI_052604_TC
20,029
18,255
1,774
91%
ESTs
Ests_SorghumDbEstSequences
227,154
215,554
11,600
95%

Collaborators

Links

Funding

This work was performed under the auspices of the US Department of Energy's Office of Science, Biological and Environmental Research Program, and by the University of California, Lawrence Berkeley National Laboratory under contract No. DE-AC02-05CH11231, Lawrence Livermore National Laboratory under Contract No. DE-AC52-07NA27344, and Los Alamos National Laboratory under contract No. DE-AC02-06NA25396.

 

Comments/Questions
DOE Joint Genome Institute © 2008