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Anaeromyxobacter Fw109-5 is a mesophilic, iron-reducing bacterium that was recently isolated from subsurface sediments (approximately 15 m) at the ERSP-FRC in Oak Ridge, TN. The groundwater at the well had a pH of 6.09 and contained approximately 1.4 mM nitrate and 0.9 µM uranium. Anaeromyxobacter are high G+C (73.5%) d-Proteobacteria distantly related to the genus Myxococcus. Based upon SSU rRNA gene sequences, the closest cultivable relative is Anaeromyxobacter dehalogenans 2CP-C with 96.5% sequence identity. The strain fw109-5 can be grown in the pH range of 4.0 to 9.0, but optimal growth was observed from pH 7.0 to 8.0. To date, known electron donors included acetate, lactate, and pyruvate, and electron acceptors included nitrate and iron(III). Optimal growth is under anaerobic conditions, and slightly microaerophilic conditions can be tolerated. The bacterium grows very slowly with ethanol, cellobiose, and starch. Yeast extract and peptone do not support growth, and the organism appears to be oligotrophic. The Anaeromyxobacter Fw109-5 genome is approximately 5.2 Mb in size with 4,336 candidate protein-coding genes.