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The images are electron micrographs of Thermosinus carboxydivorans Nor1 (DSM 14886; VKM B-2281) cells. Metal deposition is detectable in the cells during growth using FeIII reduction for energy conservation. Thin sections (a, c) and negative staining of whole cells (b). The bars are 0.5 µm. Specimens of whole cells for electron microscopy were negatively stained with 2% phosphotungstic acid. For the preparation of thin sections, cells were fixed with 5% glutaraldehyde for 2 h and 1% OsO4 for 4 h at 4 degrees C and then embedded in Epon-812. The thin sections were stained with uranyl acetate and lead citrate. Electron micrographs were taken with a JEM-100C electron microscope. |
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