Seq. Project name:
Euryarchaeota archaeon SCGC AB-637-J17
( Project ID: 1014101 )
Product:
Microbial Minimal Draft, Single Cell
Proposal Name:
Dark ocean microbial single cell genomics
(Proposal ID: 620)
Project PI:
User Program:
CSP
Program Year:
2012
Scientific Program:
Microbial
Release Date:
2023-02-25
| Organism | |
|---|---|
| Genus/species/strain/isolate: | Euryarchaeota archaeon SCGC AB-637-J17 / SCGC AB-637-J17 / SCGC AB-637-J17 |
| GOLD ID: | Gp0034075 |
| Contacts | |
|---|---|
| JGI: | [email protected] |
| Request DNA: | Ramunas Stepanauskas <[email protected]> |
| General Information | |
|---|---|
|
QD/SAG JGI SINGLE-CELL QC AND ASSEMBLY REPORT -
1014101
unclassified Euryarchaeota (miscellaneous)
1) RAW DATA:
LibraryName NumReads ReadType ReadType FileName
NOZX 32053412 2x157 Illumina Std PE (cassava 1.8) 6669.1.51253.CCAACA.fastq
2) ILLUMINA STD PE READ FILTERING STATS:
Reads were screened against human contaminants, synthetic oligos used
in the Illumina sequencing process and normalized. Pairs of matching
reads were removed from the dataset.
Total input reads: 32053412 (100%)
Num contam reads removed: 24 (0.0%)
- human_chr8 2 0.00%
- human_chr1 1 0.00%
- human_chr18 1 0.00%
- human_chr11 1 0.00%
- human_chr14 1 0.00%
- human_chr7 1 0.00%
- human_chr10 1 0.00%
- human_chr16 1 0.00%
- human_chr3 1 0.00%
- human_chr9 1 0.00%
- human_chr12 1 0.00%
- human_chr4 1 0.00%
Artifact reads removed: 138 (0.0%)
Normalized reads removed: 31911146 (99.6%)
Total reads removed: 31911308 (99.6%)
Total reads remaining: 142104 (0.4%)
3) ILLUMINA STD PE READ IDENTIFICATION STATS
This step identifies contaminants but does not remove them from the dataset.
Total input reads: 32053412 (100%)
Num contam reads identified: 2 (0.0%)
- Pseudomonas 2 0.00%
4) ASSEMBLY STATS:
a) IDBA-UD:
Avg GC Content: 31.62 +/- 6.60%
Largest Contig: 38.0 KB
Main genome scaffold total: 221
Main genome contig total: 221
Main genome scaffold sequence total: 668.8 KB
Main genome contig sequence total: 668.8 KB (-> 0.0% gap)
Main genome scaffold N/L50: 21/8.4 KB
Main genome contig N/L50: 21/8.4 KB
Number of scaffolds > 50 KB: 0
% main genome in scaffolds > 50 KB: 0.0%
Minimum Number Number Total Total Scaffold
Scaffold of of Scaffold Contig Contig
Length Scaffolds Contigs Length Length Coverage
-------- --------- ------- ----------- ----------- --------
All 221 221 668,832 668,832 100.00%
1 kb 106 106 611,259 611,259 100.00%
2.5 kb 64 64 540,250 540,250 100.00%
5 kb 39 39 451,216 451,216 100.00%
10 kb 16 16 296,321 296,321 100.00%
25 kb 3 3 95,613 95,613 100.00%
b) Allpaths + IDBA-UD simulated read pairs:
Assembly stats of the ALLPATHS assembly. The input contains simulated
1-3 kb read pairs created from the IDBA-UD assembly and reads that
have been filtered for contamination, artifacts and normalized.
Avg GC Content: 29.78 +/- 5.23%
Largest Contig: 140.6 KB
Main genome scaffold total: 43
Main genome contig total: 43
Main genome scaffold sequence total: 589.1 KB
Main genome contig sequence total: 589.1 KB (-> 0.0% gap)
Main genome scaffold N/L50: 6/29.7 KB
Main genome contig N/L50: 6/29.7 KB
Number of scaffolds > 50 KB: 1
% main genome in scaffolds > 50 KB: 23.9%
Minimum Number Number Total Total Scaffold
Scaffold of of Scaffold Contig Contig
Length Scaffolds Contigs Length Length Coverage
-------- --------- ------- ----------- ----------- --------
All 43 43 589,131 589,131 100.00%
1 kb 43 43 589,131 589,131 100.00%
2.5 kb 37 37 577,385 577,385 100.00%
5 kb 27 27 544,226 544,226 100.00%
10 kb 17 17 474,216 474,216 100.00%
25 kb 6 6 300,129 300,129 100.00%
50 kb 1 1 140,620 140,620 100.00%
100 kb 1 1 140,620 140,620 100.00%
5) KEY PIPELINE CMDS:
a) Contamination removal step:
Bwa version: 0.5.9-r16
Bwa aln params:
Bwa sampe params: -A -P -s
b) Artifact removal step:
duk params: -k 22 -s 1 -c 1
c) Normalization step:
kmernorm params: -k 21 -t 15 -c 2
d) IDBA-UD assembly step for creating simulated read pairs:
IDBA-UD version: 1.0.9
IDBA-UD params: --no_local
e) ALLPATHS assembly step:
ALLPATHS version: r42328
Contents of in_libs.csv:
library_name, project_name, organism_name, type, paired, frag_size, frag_stddev, insert_size, insert_stddev, read_orientation, genomic_start, genomic_end
STD_1,project,assembly,fragment,1,260,35,,,inward,0,0
SIMREADS,project,assembly,jumping,1,,,3000,300,inward,0,0
6) WORKFLOW STEPS:
1. Removed contamination (human contaminants).
2. Removed illumina artifacts (synthetic oligos used in the laboratory).
3. Normalized read coverage.
4. Created IDBA-UD assembly of the contam+artifact+normalized filtered data.
5. Created simulated 1-3 kb read pairs using IDBA-UD contigs from step 4.
6. Created allpaths assembly using IDBA-UD simulated read pairs (step 5) and the contam+artifact+normalized filtered data.
7) ASSEMBLERS USED:
IDBA-UD, AllpathsLG
8) RELEASE DATE:
Thu Apr 4 10:57:58 PDT 2013 By Alexander Spunde- [email protected]
9) AUTHORS:
For additional information, please contact:
Stephan Trong - [email protected]
James Han - [email protected]
This file was automatically generated by the jigsaw pipeline software (version 2.1.2).
|
| Funding | |
|---|---|
| The work conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. | |
