Seq. Project name:
Euryarchaeota archaeon SCGC AC-312-F18
( Project ID: 1014107 )
Product:
Microbial Minimal Draft, Single Cell
Proposal Name:
Dark ocean microbial single cell genomics
(Proposal ID: 620)
Project PI:
User Program:
CSP
Program Year:
2012
Scientific Program:
Microbial
Release Date:
2017-12-13
Organism | |
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Genus/species/strain/isolate: | Euryarchaeota archaeon SCGC AC-312-F18 / SCGC AC-312-F18 / SCGC AC-312-F18 |
GOLD ID: | Gp0034074 |
Contacts | |
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JGI: | [email protected] |
Request DNA: | Ramunas Stepanauskas <[email protected]> |
General Information | |
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QD/SAG JGI SINGLE-CELL QC AND ASSEMBLY REPORT -
1014107
unclassified Euryarchaeota (miscellaneous)
1) RAW DATA:
LibraryName NumReads ReadType ReadType FileName
LIB 30547928 2x157 Illumina Std PE (cassava 1.8) 6669.3.51255.AAACAT.fastq
2) ILLUMINA STD PE READ FILTERING STATS:
Reads were screened against human contaminants, synthetic oligos used
in the Illumina sequencing process and normalized. Pairs of matching
reads were removed from the dataset.
Total input reads: 30547928 (100%)
Num contam reads removed: 0 (0.0%)
Artifact reads removed: 721560 (2.4%)
Normalized reads removed: 29730794 (97.3%)
Total reads removed: 30452354 (99.7%)
Total reads remaining: 95574 (0.3%)
3) ILLUMINA STD PE READ IDENTIFICATION STATS
This step identifies contaminants but does not remove them from the dataset.
Total input reads: 30547928 (100%)
Num contam reads identified: 4 (0.0%)
- Pseudomonas 2 0.00%
- Shigella 2 0.00%
4) ASSEMBLY STATS:
a) IDBA-UD:
Avg GC Content: 34.72 +/- 6.79%
Largest Contig: 47.3 KB
Main genome scaffold total: 153
Main genome contig total: 153
Main genome scaffold sequence total: 440.4 KB
Main genome contig sequence total: 440.4 KB (-> 0.0% gap)
Main genome scaffold N/L50: 10/12.7 KB
Main genome contig N/L50: 10/12.7 KB
Number of scaffolds > 50 KB: 0
% main genome in scaffolds > 50 KB: 0.0%
Minimum Number Number Total Total Scaffold
Scaffold of of Scaffold Contig Contig
Length Scaffolds Contigs Length Length Coverage
-------- --------- ------- ----------- ----------- --------
All 153 153 440,412 440,412 100.00%
1 kb 59 59 394,523 394,523 100.00%
2.5 kb 35 35 355,562 355,562 100.00%
5 kb 21 21 299,000 299,000 100.00%
10 kb 11 11 234,413 234,413 100.00%
25 kb 4 4 126,241 126,241 100.00%
b) Allpaths + IDBA-UD simulated read pairs:
Assembly stats of the ALLPATHS assembly. The input contains simulated
1-3 kb read pairs created from the IDBA-UD assembly and reads that
have been filtered for contamination, artifacts and normalized.
Avg GC Content: 32.82 +/- 2.37%
Largest Contig: 106.3 KB
Main genome scaffold total: 9
Main genome contig total: 9
Main genome scaffold sequence total: 377.9 KB
Main genome contig sequence total: 377.9 KB (-> 0.0% gap)
Main genome scaffold N/L50: 3/58.5 KB
Main genome contig N/L50: 3/58.5 KB
Number of scaffolds > 50 KB: 4
% main genome in scaffolds > 50 KB: 77.1%
Minimum Number Number Total Total Scaffold
Scaffold of of Scaffold Contig Contig
Length Scaffolds Contigs Length Length Coverage
-------- --------- ------- ----------- ----------- --------
All 9 9 377,942 377,942 100.00%
1 kb 9 9 377,942 377,942 100.00%
2.5 kb 8 8 375,484 375,484 100.00%
5 kb 6 6 366,807 366,807 100.00%
10 kb 6 6 366,807 366,807 100.00%
25 kb 6 6 366,807 366,807 100.00%
50 kb 4 4 291,356 291,356 100.00%
100 kb 1 1 106,348 106,348 100.00%
5) KEY PIPELINE CMDS:
a) Contamination removal step:
Bwa version: 0.5.9-r16
Bwa aln params:
Bwa sampe params: -A -P -s
b) Artifact removal step:
duk params: -k 22 -s 1 -c 1
c) Normalization step:
kmernorm params: -k 21 -t 15 -c 2
d) IDBA-UD assembly step for creating simulated read pairs:
IDBA-UD version: 1.0.9
IDBA-UD params: --no_local
e) ALLPATHS assembly step:
ALLPATHS version: r42328
Contents of in_libs.csv:
library_name, project_name, organism_name, type, paired, frag_size, frag_stddev, insert_size, insert_stddev, read_orientation, genomic_start, genomic_end
STD_1,project,assembly,fragment,1,260,35,,,inward,0,0
SIMREADS,project,assembly,jumping,1,,,3000,300,inward,0,0
6) WORKFLOW STEPS:
1. Removed contamination (human contaminants).
2. Removed illumina artifacts (synthetic oligos used in the laboratory).
3. Normalized read coverage.
4. Created IDBA-UD assembly of the contam+artifact+normalized filtered data.
5. Created simulated 1-3 kb read pairs using IDBA-UD contigs from step 4.
6. Created allpaths assembly using IDBA-UD simulated read pairs (step 5) and the contam+artifact+normalized filtered data.
7) ASSEMBLERS USED:
IDBA-UD, AllpathsLG
8) RELEASE DATE:
Mon Apr 8 15:16:12 PDT 2013 By Alexander Spunde- [email protected]
9) AUTHORS:
For additional information, please contact:
Stephan Trong - [email protected]
James Han - [email protected]
This file was automatically generated by the jigsaw pipeline software (version 2.1.2.1).
|
Funding | |
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The work conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. |