Seq. Project name:
Planctomycetes bacterium SCGC AAA282-C19
( Project ID: 1014137 )
Product:
Microbial Minimal Draft, Single Cell
Proposal Name:
Dark ocean microbial single cell genomics
(Proposal ID: 620)
Project PI:
User Program:
CSP
Program Year:
2012
Scientific Program:
Microbial
Release Date:
2017-12-13
Organism | |
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Genus/species/strain/isolate: | Planctomycetes bacterium SCGC AAA282-C19 / SCGC AAA282-C19 / SCGC AAA282-C19 |
GOLD ID: | Gp0033584 |
Contacts | |
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JGI: | [email protected] |
Request DNA: | Ramunas Stepanauskas <[email protected]> |
General Information | |
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QD/SAG JGI SINGLE-CELL QC AND ASSEMBLY REPORT -
1014137
unclassified Planctomycetes
1) RAW DATA:
LibraryName NumReads ReadType ReadType FileName
NPAS 21168436 2x150 Illumina Std PE (cassava 1.8) 6668.1.51104.GTAGAG.fastq
2) ILLUMINA STD PE READ FILTERING STATS:
Reads were screened against human contaminants, synthetic oligos used
in the Illumina sequencing process and normalized. Pairs of matching
reads were removed from the dataset.
Total input reads: 21168436 (100%)
Num contam reads removed: 14 (0.0%)
- human_chr2 5 0.00%
- human_chr22 1 0.00%
- human_chr12 1 0.00%
Artifact reads removed: 2 (0.0%)
Normalized reads removed: 20962126 (99.0%)
Total reads removed: 20962142 (99.0%)
Total reads remaining: 206294 (1.0%)
3) ILLUMINA STD PE READ IDENTIFICATION STATS
This step identifies contaminants but does not remove them from the dataset.
Total input reads: 21168436 (100%)
Num contam reads identified: 0 (0.0%)
4) ASSEMBLY STATS:
a) IDBA-UD:
Avg GC Content: 43.30 +/- 5.63%
Largest Contig: 49.4 KB
Main genome scaffold total: 392
Main genome contig total: 392
Main genome scaffold sequence total: 1.1 MB
Main genome contig sequence total: 1.1 MB (-> 0.0% gap)
Main genome scaffold N/L50: 34/8.4 KB
Main genome contig N/L50: 34/8.4 KB
Number of scaffolds > 50 KB: 0
% main genome in scaffolds > 50 KB: 0.0%
Minimum Number Number Total Total Scaffold
Scaffold of of Scaffold Contig Contig
Length Scaffolds Contigs Length Length Coverage
-------- --------- ------- ----------- ----------- --------
All 392 392 1,060,955 1,060,955 100.00%
1 kb 177 177 946,464 946,464 100.00%
2.5 kb 106 106 838,014 838,014 100.00%
5 kb 55 55 666,537 666,537 100.00%
10 kb 26 26 464,463 464,463 100.00%
25 kb 3 3 132,938 132,938 100.00%
b) Allpaths + IDBA-UD simulated read pairs:
Assembly stats of the ALLPATHS assembly. The input contains simulated
1-3 kb read pairs created from the IDBA-UD assembly and reads that
have been filtered for contamination, artifacts and normalized.
Avg GC Content: 44.64 +/- 3.39%
Largest Contig: 81.1 KB
Main genome scaffold total: 71
Main genome contig total: 71
Main genome scaffold sequence total: 873.2 KB
Main genome contig sequence total: 873.2 KB (-> 0.0% gap)
Main genome scaffold N/L50: 11/22.0 KB
Main genome contig N/L50: 11/22.0 KB
Number of scaffolds > 50 KB: 3
% main genome in scaffolds > 50 KB: 23.8%
Minimum Number Number Total Total Scaffold
Scaffold of of Scaffold Contig Contig
Length Scaffolds Contigs Length Length Coverage
-------- --------- ------- ----------- ----------- --------
All 71 71 873,207 873,207 100.00%
1 kb 71 71 873,207 873,207 100.00%
2.5 kb 63 63 855,490 855,490 100.00%
5 kb 43 43 785,106 785,106 100.00%
10 kb 24 24 651,108 651,108 100.00%
25 kb 10 10 432,923 432,923 100.00%
50 kb 3 3 208,244 208,244 100.00%
5) KEY PIPELINE CMDS:
a) Contamination removal step:
Bwa version: 0.5.9-r16
Bwa aln params:
Bwa sampe params: -A -P -s
b) Artifact removal step:
duk params: -k 22 -s 1 -c 1
c) Normalization step:
kmernorm params: -k 21 -t 15 -c 2
d) IDBA-UD assembly step for creating simulated read pairs:
IDBA-UD version: 1.0.9
IDBA-UD params: --no_local
e) ALLPATHS assembly step:
ALLPATHS version: r42328
Contents of in_libs.csv:
library_name, project_name, organism_name, type, paired, frag_size, frag_stddev, insert_size, insert_stddev, read_orientation, genomic_start, genomic_end
STD_1,project,assembly,fragment,1,260,35,,,inward,0,0
SIMREADS,project,assembly,jumping,1,,,3000,300,inward,0,0
6) WORKFLOW STEPS:
1. Removed contamination (human contaminants).
2. Removed illumina artifacts (synthetic oligos used in the laboratory).
3. Normalized read coverage.
4. Created IDBA-UD assembly of the contam+artifact+normalized filtered data.
5. Created simulated 1-3 kb read pairs using IDBA-UD contigs from step 4.
6. Created allpaths assembly using IDBA-UD simulated read pairs (step 5) and the contam+artifact+normalized filtered data.
7) ASSEMBLERS USED:
IDBA-UD, AllpathsLG
8) RELEASE DATE:
02/22/2013
9) AUTHORS:
For additional information, please contact:
NAME_OF_ANALYST - [email protected]
Stephan Trong - [email protected]
James Han - [email protected]
This file was automatically generated by the jigsaw pipeline software (version 2.1.2).
|
Funding | |
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The work conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. |
Groups |
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## | Name | Type |
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1 | Planctomycetes |