Announcements
- January 23-24, 2012
CSP 2012 PI Workshop, Walnut Creek, CA - January 19-20, 2012
Microbial Genome Reannotation Workshop, Walnut Creek, CA
Releases
- January 31, 2012
Clostridium sp. BNL1100 - December 1, 2011
Acidovorax avenae avenae ATCC 19860 - December 1, 2011
Alicycliphilus denitrificans K601 - December 1, 2011
Cellulosilyticum lentocellum RHM5, DSM 5427 - December 1, 2011
Delftia sp. Cs1-4
Single Cell Genomics
The advent of the single-cell sequencing approach opened a large window of opportunity to access the coding potential of uncultured microbes critical in environmental processes, such as carbon cycling and bioremediation, providing a powerful complement to metagenomics and metatranscriptomics. JGI's currently ongoing single cell sequencing portfolio represent uncultured bacteria and archaea from highly diverse and DOE-relevant environments including hot spring sediments, hydrothermal vents, soil, mesopelagic, sub-surface and plant-associated sites, with many sites strategically overlapping with our metagenome project portfolio.
Key goals of JGI's single cell genomics team are to:
- Develop and maintain a stable pipeline for sequencing and analysis of single cells.
- Determine if samples containing fixed cells are suitable for single cell sequencing.
- Implement techniques for sequencing mRNA from single bacterial and archaeal cells.
- Test selective sorting methods to improve our understanding of uncultured microbes beyond just genome sequence.
- Expand the Genomic Encyclopedia of Bacteria and Archaea by targeting single cell representatives of candidate phyla.
