An Error Occurred:
javax.el.ELException: /programs/metagenomes/left-side.xhtml @40,76 value="#{metagenomes_home.latestReleases}": Error reading 'latestReleases' on type gov.doe.jgi.portal.view.pages.MetagenomesHome$Proxy$_$$_WeldClientProxy
javax.faces.FacesException: javax.el.ELException: /programs/metagenomes/left-side.xhtml @40,76 value="#{metagenomes_home.latestReleases}": Error reading 'latestReleases' on type gov.doe.jgi.portal.view.pages.MetagenomesHome$Proxy$_$$_WeldClientProxy
at javax.faces.component.UIOutput.getValue(UIOutput.java:176)
at com.sun.faces.renderkit.html_basic.HtmlBasicInputRenderer.getValue(HtmlBasicInputRenderer.java:189)
at com.sun.faces.renderkit.html_basic.HtmlBasicRenderer.getCurrentValue(HtmlBasicRenderer.java:320)
at com.sun.faces.renderkit.html_basic.HtmlBasicRenderer.encodeEnd(HtmlBasicRenderer.java:200)
at javax.faces.component.UIComponentBase.encodeEnd(UIComponentBase.java:836)
at javax.faces.component.UIComponent.encodeAll(UIComponent.java:896)
at javax.faces.component.UIComponent.encodeAll(UIComponent.java:892)
at com.sun.facelets.FaceletViewHandler.renderView(FaceletViewHandler.java:592)
at gov.doe.jgi.portal.view.common.DefaultViewHandler.renderView(DefaultViewHandler.java:284)
at org.ajax4jsf.application.ViewHandlerWrapper.renderView(ViewHandlerWrapper.java:100)
at org.ajax4jsf.application.AjaxViewHandler.renderView(AjaxViewHandler.java:176)
at com.sun.faces.lifecycle.RenderResponsePhase.execute(RenderResponsePhase.java:106)
at com.sun.faces.lifecycle.LifecycleImpl.phase(LifecycleImpl.java:251)
at com.sun.faces.lifecycle.LifecycleImpl.render(LifecycleImpl.java:144)
at javax.faces.webapp.FacesServlet.service(FacesServlet.java:245)
at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:290)
at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:206)
at org.ajax4jsf.webapp.BaseXMLFilter.doXmlFilter(BaseXMLFilter.java:206)
at org.ajax4jsf.webapp.BaseFilter.handleRequest(BaseFilter.java:295)
at org.ajax4jsf.webapp.BaseFilter.processUploadsAndHandleRequest(BaseFilter.java:393)
at org.ajax4jsf.webapp.BaseFilter.doFilter(BaseFilter.java:520)
at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:235)
at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:206)
at gov.doe.jgi.portal.view.log.LogFilter.doFilter(LogFilter.java:82)
at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:235)
at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:206)
at gov.doe.jgi.portal.view.common.StaticContentFilter.doFilter(StaticContentFilter.java:167)
at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:235)
at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:206)
at gov.doe.jgi.portal.framework.data.EntityManagerFilter.doFilter(EntityManagerFilter.java:31)
at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:235)
at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:206)
at gov.doe.jgi.portal.framework.config.ConfigFilter.doFilter(ConfigFilter.java:27)
at org.apache.catalina.core.ApplicationFilterChain.internalDoFilter(ApplicationFilterChain.java:235)
at org.apache.catalina.core.ApplicationFilterChain.doFilter(ApplicationFilterChain.java:206)
at org.apache.catalina.core.StandardWrapperValve.invoke(StandardWrapperValve.java:233)
at org.apache.catalina.core.StandardContextValve.invoke(StandardContextValve.java:191)
at org.apache.catalina.core.StandardHostValve.invoke(StandardHostValve.java:127)
at org.apache.catalina.valves.ErrorReportValve.invoke(ErrorReportValve.java:102)
at gov.doe.jgi.portal.tomcat.SessionInvalidateValve.invoke(SessionInvalidateValve.java:74)
at org.apache.catalina.core.StandardEngineValve.invoke(StandardEngineValve.java:109)
at org.apache.catalina.connector.CoyoteAdapter.service(CoyoteAdapter.java:298)
at org.apache.jk.server.JkCoyoteHandler.invoke(JkCoyoteHandler.java:190)
at org.apache.jk.common.HandlerRequest.invoke(HandlerRequest.java:291)
at org.apache.jk.common.ChannelSocket.invoke(ChannelSocket.java:774)
at org.apache.jk.common.ChannelSocket.processConnection(ChannelSocket.java:703)
at org.apache.jk.common.ChannelSocket$SocketConnection.runIt(ChannelSocket.java:896)
at org.apache.tomcat.util.threads.ThreadPool$ControlRunnable.run(ThreadPool.java:690)
at java.lang.Thread.run(Thread.java:722)
Caused by: javax.el.ELException: /programs/metagenomes/left-side.xhtml @40,76 value="#{metagenomes_home.latestReleases}": Error reading 'latestReleases' on type gov.doe.jgi.portal.view.pages.MetagenomesHome$Proxy$_$$_WeldClientProxy
at com.sun.facelets.el.TagValueExpression.getValue(TagValueExpression.java:76)
at javax.faces.component.UIOutput.getValue(UIOutput.java:173)
... 48 more
Caused by: java.lang.NullPointerException
at java.util.regex.Matcher.getTextLength(Matcher.java:1233)
at java.util.regex.Matcher.reset(Matcher.java:308)
at java.util.regex.Matcher.<init>(Matcher.java:228)
at java.util.regex.Pattern.matcher(Pattern.java:1088)
at gov.doe.jgi.portal.framework.config.PortalConfig.getOrganismsMatching(PortalConfig.java:1012)
at gov.doe.jgi.portal.view.pages.ProgramHome$ProgramHomeData.init(ProgramHome.java:124)
at gov.doe.jgi.portal.view.pages.ProgramHome$ProgramHomeData.check(ProgramHome.java:114)
at gov.doe.jgi.portal.view.pages.ProgramHome$ProgramHomeData.getLatestReleases(ProgramHome.java:74)
at gov.doe.jgi.portal.view.pages.ProgramHome.getLatestReleases(ProgramHome.java:224)
at gov.doe.jgi.portal.view.pages.MetagenomesHome$Proxy$_$$_WeldClientProxy.getLatestReleases(MetagenomesHome$Proxy$_$$_WeldClientProxy.java)
at sun.reflect.GeneratedMethodAccessor2367.invoke(Unknown Source)
at sun.reflect.DelegatingMethodAccessorImpl.invoke(DelegatingMethodAccessorImpl.java:43)
at java.lang.reflect.Method.invoke(Method.java:601)
at javax.el.BeanELResolver.getValue(BeanELResolver.java:62)
at javax.el.CompositeELResolver.getValue(CompositeELResolver.java:54)
at com.sun.faces.el.FacesCompositeELResolver.getValue(FacesCompositeELResolver.java:64)
at org.apache.el.parser.AstValue.getValue(AstValue.java:123)
at org.apache.el.ValueExpressionImpl.getValue(ValueExpressionImpl.java:186)
at org.jboss.weld.el.WeldValueExpression.getValue(WeldValueExpression.java:50)
at com.sun.facelets.el.TagValueExpression.getValue(TagValueExpression.java:71)
... 49 more
- <AjaxViewRoot id="_viewRoot" immediate="false" locale="en_US" renderKitId="HTML_BASIC" renderRegionOnly="false" rendered="true" selfRendered="false" submitted="false" transient="false" viewId="/programs/metagenomes/data_reduction.xhtml">
- <!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd" >
- <html xmlns="http://www.w3.org/1999/xhtml" xmlns:t="http://myfaces.apache.org/tomahawk">
<head>
- <HtmlOutputText escape="false" id="j_id2" rendered="true" transient="false" value="<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1"/>
<link href="/css/genome2.css" rel="stylesheet" type="text/css"/>
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- <script type="text/javascript" src="/js/jquery.js"/>
<script type="text/javascript" src="/js/treeUtils.js"/>
<script type="text/javascript" src="/js/preload.js"/>
<script type="text/javascript" src="/js/superfish.js"/>
<script type="text/javascript" src="/js/supersubs.js"/>
<script type="text/javascript">
<!--
jQuery.noConflict();
var g_selectOrg = null;
var g_wasInitialized = false;
function gotoOrganism() {
if( !g_wasInitialized )
initOrgSelectionBoxes();
var selectLocation = document.getElementById('selectLocation');
var encodedString = g_selectOrg.options[g_selectOrg.selectedIndex].value;
var loc = selectLocation.options[selectLocation.selectedIndex].value;
var url = "error";
if( loc == "home" )
url = encodedString.replace(/(.*),(.*),(.*),(.*)/,'/$2');
else if( loc == "browse" )
url = encodedString.replace(/(.*),(.*),(.*),(.*)/,'/cgi-bin/browserLoad?db=$2&position=$3');
else if( loc == "blast" )
url = encodedString.replace(/(.*),(.*),(.*),(.*)/,'/pages/blast.jsf?db=$2');
else if( loc == "download" )
url = encodedString.replace(/(.*),(.*),(.*),(.*)/,'/$2/$2.download.html');
else if( loc == "annotation" )
url = encodedString.replace(/(.*),(.*),(.*),(.*)/,'/$2/$2.annotation.html');
//alert('redirecting to ' + url);
top.location.href = url;
}
function emailCollaborator() {
var selectEmail = document.getElementById("selectEmail");
var emailObj = selectEmail.options[ selectEmail.selectedIndex ];
location.href = "mailto:" + emailObj.value.replace(/\+/g, "%20") + "?subject=" + escape(emailObj.text);
}
function filterOrganisms() {
if( !g_wasInitialized )
initOrgSelectionBoxes();
var category = jQuery("#selectCategory").val();
var selectOrg;
if( category == "all" )
selectOrg = jQuery( "#selectOrg" );
else if( category == "Bacteria" )
selectOrg = jQuery( "#selectOrgBacteria" );
else if( category == "Eukaryota" )
selectOrg = jQuery( "#selectOrgEukaryota" );
else if( category == "Archaea" )
selectOrg = jQuery( "#selectOrgArchaea" );
else if( category == "Microbiome" )
selectOrg = jQuery( "#selectOrgMicrobiomes" );
g_selectOrg = selectOrg[0];
selectOrg[0].selectedIndex = 0;
jQuery(".orgSelectBox").hide();
selectOrg.show();
filterLocations(selectOrg[0]);
}
function filterLocations(selectOrg) {
if( !g_wasInitialized )
initOrgSelectionBoxes();
var sel = selectOrg.options[selectOrg.selectedIndex];
var isEuk = sel.value.indexOf("Eukaryota") == 0;
var selectedLocation = jQuery( "#selectLocation" )[0];
if( !isEuk ) {
var showAnnotation = sel.value[sel.value.length - 1] == '1';
if( showAnnotation ) {
selectedLocation.options[1].text = "Annotation";
selectedLocation.options[1].value = "annotation";
selectedLocation.options[1].disabled = false;
} else {
if( selectedLocation.selectedIndex == 1 ) // Browse
selectedLocation.selectedIndex = 0; // Home
var browseLoc = selectedLocation.options[1];
browseLoc.disabled = true;
}
} else {
selectedLocation.options[1].text = "Browse";
selectedLocation.options[1].value = "browse";
selectedLocation.options[1].disabled = false;
}
}
function initOrgSelectionBoxes() {
var selectCategory = jQuery( "#selectCategory" );
if( selectCategory.length == 0 ) // if the page is rendered without the organism drop-downs, then selectOrg.length will be 0
return;
g_wasInitialized = true;
jQuery("#selectCategory").change( function () { filterOrganisms(); });
jQuery(".orgSelectBox").change( function () { filterLocations(this); });
filterOrganisms(); // initialize
}
jQuery(document).ready(function(){
jQuery("ul.sf-menu").supersubs({
minWidth: 12, // minimum width of sub-menus in em units
maxWidth: 20, // maximum width of sub-menus in em units
extraWidth: 1 // extra width can ensure lines don't sometimes turn over
// due to slight rounding differences and font-family
}).superfish(); // call supersubs first, then superfish, so that subs are
// not display:none when measuring. Call before initialising
// containing tabs for same reason.
});
var treeOfLife = new Object;
function showTreeOfLifeNodeMenu(nodeName, nodeLabel) {
var url = '/ext-api/tree-of-life/metagenome-node/' + nodeName;
// The 'menu' array will be filled in with data during the eval(data) call below.
if( typeof menu[nodeName + ".menuindex"] != 'number' || typeof menu[nodeName + ".Name"] != 'string' || menu[nodeName + ".menuindex"] < 1) {
jQuery.get(url, function(data) {
treeOfLife[nodeName] = 1;
eval(data);
multitool( x, y, nodeName, nodeLabel );
});
} else {
multitool( x, y, nodeName, nodeLabel );
}
}
-->
</script>
<script type="text/javascript">
function init() {
MM_preloadImages('/images/closeBtn_over.gif','/images/questionBtn_over.gif');
initOrgSelectionBoxes();
}
</script>
<link href="/css/tree.css" rel="stylesheet" type="text/css"/>
<link href="/css/search.css" rel="stylesheet" type="text/css"/>
<link href="/css/metagenome.css" rel="stylesheet" type="text/css"/>
<link rel="stylesheet" media="screen" href="/css/superfish.css"/>
<link rel="stylesheet" media="screen" href="/css/superfish_metagenome.css"/>
- <title>Metagenome gene prediction & data reduction</title>
</head>
<body onload="init();">
- <HtmlOutputText escape="false" id="j_id5" rendered="true" transient="false" value="<div class="header_wrap">
<div class="header_top"><div id="subnavleft"><a class="skipnav" href="#skipnav" accesskey="S"><img src="/images/clear.gif" alt="skip navigation" width="1" height="1" border="0" /></a><ul id="subnavicons"><li><a target="_parent" href="http://www.jgi.doe.gov" alt=""> <img class="header_image" alt="DOE Joint Genome Institute logo" style="padding-right: 2em" src="/images/jgiLogo_icon.gif"/></a></li><li><a target="_parent" href="/"><img border="0" alt="Genome Portal icon" src="/images/tree_icon.gif"/></a></li>
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- <!--ui:define name="body"-->
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<div id="toolmenudiv" style="position: absolute; padding:0;margin:0; left: 100px; top: 100px; visibility: visible; overflow: auto;" onmouseover="window.clearTimeout(timeoutId);" onmouseout="timeoutId = window.setTimeout('divoff(\'toolmenudiv\')', APP_TIMEOUT);"/>
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<div id="container">
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- <div id="nav">
<ul class="sf-menu">
<li class="#{pagetype=='home'?'selected':''}"><a href="/programs/metagenomes/index.jsf" title="Back to home page" class="topmenu" id="home">Home</a></li>
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<li class="#{pagetype=='science'?'selected':''}"><a href="/programs/metagenomes/publications_genomes.jsf" class="topmenu" title="Metagenome Projects and Publications" id="science">Science</a>
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<li><a href="/programs/metagenomes/assembly.jsf">Assembly</a></li>
<li><a href="http://journals.sfu.ca/sigen/index.php/sigen/article/viewFile/sigs.632/sigs632.pdf">Annotation Pipeline</a></li>
<li><a href="http://clams.jgi-psf.org/">CLaMS</a></li>
<li><a href="http://coal.jgi-psf.org/CoverageServer/">GammaCov</a></li>
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</ul>
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<li><a class="sf-with-ul" href="/programs/metagenomes/benchmarks.jsf">Benchmarks</a></li>
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<ul>
<li><a href="http://fames.jgi-psf.org/">FAMeS</a></li>
<li><a href="/programs/metagenomes/data_reduction.jsf">Data Reduction</a></li>
<li><a href="/programs/metagenomes/binning.jsf">Binning</a></li>
<li><a href="/programs/metagenomes/cow_rumen_analysis.jsf">Cow Rumen Metagenome Data Analysis</a></li>
</ul>
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</ul>
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<ul class="submenu">
<li><a class="sf-with-ul" href="/programs/metagenomes/workshops.jsf">Workshops</a></li>
<li><a class="sf-with-ul" href="/programs/metagenomes/meetings.jsf">Meetings</a></li>
</ul>
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<li class="#{pagetype=='about_program'?'selected':''}"><a href="/programs/metagenomes/mission.jsf" title="about program, mission, organization, advisory_committee" class="topmenu" id="about_program">About Program</a>
<ul class="submenu">
<li><a class="sf-with-ul" href="/programs/metagenomes/mission.jsf">Mission</a></li>
<li><a class="sf-with-ul" href="/programs/metagenomes/organization.jsf">Organization</a></li>
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</ul>
</div>
<img src="/images/metagenome/metagenome_banner.png" width="" height="" border="0" usemap="#logomap" class="banner" alt="banner with logo of Metagenomics program"/>
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<area shape="rect" coords="10,0,350,72" href="/programs/metagenomes/index.jsf" alt="Metagenomics Program at JGI, Home"/>
</map>
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- <!--end of navigation and top banner-->
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<a href="/pages/fungi/about_program.jsf"><h2 class="list_title" >Genomic Encyclopedia of Fungi</h2></a>
<ul>
<li><a class="list_header_feedstock" href="/pages/fungi/about_program.jsf#feedstock">Plant Feedstock Health</a></li>
<li><a class="list_subheader" href="/pages/fungi/about_program.jsf#symbiosis">Mycorrhizal Symbiosis</a></li>
<li><a class="list_subheader" href="/pages/fungi/about_program.jsf#pathogenicity">Plant Pathogenicity</a></li>
<li><a class="list_subheader" href="/pages/fungi/about_program.jsf#biocontrol">Biocontrol</a></li>
<li><a class="list_header_biorefinery" href="/pages/fungi/about_program.jsf#biorefinery">Biorefinery</a></li>
<li><a class="list_subheader" href="/pages/fungi/about_program.jsf#biorefinery">Lignocellulose Degradation</a></li>
<li><a class="list_subheader" href="/pages/fungi/about_program.jsf#biorefinery">Sugar Fermentation</a></li>
<li><a class="list_subheader" href="/pages/fungi/about_program.jsf#biorefinery">Industrial Organisms</a></li>
<li><a class="list_header_diversity" href="/pages/fungi/about_program.jsf#diversity">Fungal Diversity</a></li>
</ul>
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<div class="clear"/>
<!--Announcements -->
<div id="announcements">
<h2 class="red">Announcements</h2>
<ul>
<li><span class="date">January 23-24, 2012 <br/>
</span><a href="http://www.jgi.doe.gov/meetings/csp-pi-workshop/">CSP 2012 PI Workshop</a>, Walnut Creek, CA
</li>
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<h2 class="red">Releases</h2>
- <HtmlOutputText escape="false" id="j_id10" rendered="true" transient="false"/>
- <a class="link_arrow" href="/programs/metagenomes/genome-releases.jsf">more</a>
<!--end of New Releases -->
<div class="grey_divider"/>
</div>
</div>
- <!--end of left side panel -->
<!--right side panel -->
<div id="rightside">
<h1>Data Reduction</h1>
<h3>Motivation</h3>
<p> New sequencing technologies are providing an unprecedented quantity of sequence data, but they also pose new challenges for accurate and efficient processing of that data. Their short read length and the presence of sequencing errors <b>decrease the accuracy of <i>ab initio</i> gene prediction</b>. Similarity-based gene prediction methods are not affected by these limitations, but these methods are computationally intensive and are limited to the detection of protein-coding genes with sequence similarity to known genes. Furthermore, the typical metagenome dataset contains an extremely large number of sequences making the analysis a difficult task for any researcher. This project has employed simulated datasets to compare the performance of several gene prediction methods that are widely applied to metagenome data sets.</p>
<h3>Results</h3>
<p><b><i>Ab initio</i></b> gene callers are sensitive to the size and the quality of the metagenome sequences. Small size sequences, even without any errors, do not contain enough information for accurate gene prediction resulting in a large number of missed genes. Overall, the performance of these tools is very good on sequences without errors. However, on sequences with errors the performance drops significantly resulting in error rates larger than 20%. Similarity based methods avoid this high error rate but with significant increase in computational time and loss of detection of sequences with low similarity to what is already available in the public databases. Based on these results, a <b>gene calling pipeline</b> was developed the Microbial Genome Analysis Group at JGI. This pipeline employs a similarity based approach, <b>Multiblastx</b>, in order to predict genes on short sequences (<800bp) and ab initio methods for longer sequences. Also ab initio gene calling is used to predict genes in the intergenic regions of sequences with size between 300 and 800 bp after genes have been predicted with multiblastx.</p>
<p>A data compression method, Metafolding, that can be employed as a post-processing step for metagenome datasets has been developed by the Microbial Genome Analysis Group at JGI. Metafolding achieves close to 40% data compression without significant loss of functional and phylogenetic information. </p>
<p> Multiblastx and Metafolding are already employed for the annotation of metagenome datasets prior to their integration into the IMG/M ER metagenome curation and comparative analysis system. A draft paper describing this R&D project has been prepared and is currently undergoing review before submission to a peer reviewed journal.</p>
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