Pseudomonas putida GB-1
   
   
 

Pseudomonas putida strain GB-1, a fresh water, Gram-negative gamma-proteobacterium, is a genetically tractable, robust manganese (Mn) oxidizer, and as such, is an ideal model for unraveling the catalytic mechanism for, and the molecular regulation of Mn(IV) oxide production and its eventual accumulation on the cell surface at the onset of stationary phase. Since its isolation from Green Bay nearly 20 years ago by Ken Nealson’s group (then at the Center for Great Lakes Studies, Univ. Wisconsin-Milwaukee), it has been the non spore-forming, model organism (along with the closely-related strain MnB1) for molecular genetic studies of Mn(II) oxidization, protein transport and biofilm formation and for biochemical studies on protein purification and Mn(III)-pyoverdine binding.

References

Brouwers, G.-J., J. P. M. de Vrind, P. L. A. M. Corstjens, P. Cornelis, C. Baysse and E. W. de Vrind-de Jong (1999). "CumA, a gene encoding a multicopper oxidase, is involved in Mn2+-oxidation in Pseudomonas putida GB-1." Applied and Environmental Microbiology 65: 1762-1768.

Brouwers, G.-J., J. P. M. de Vrind, P. L. A. M. Corstjens, P. Westbroek and E. W. de Vrind-de Jong (1997). Pseudomonas genes involved in the oxidation of manganese. Abstracts, Geological Society of America Annual Meeting. Salt Lake City, UT.

Brouwers, G. J., E. W. de Vrind-De Jong, P. L. A. M. Corstjens and E. W. V. Jong (1998). "Involvement of genes of the two-step protein secretion pathway in the transport of the manganese-oxidizing factor across the outer membrane of Pseudomonas putida strain GB-I." American Mineralogist 83: 1573-1582.

Brouwers, G.-J., E. Vijgenboom, P. L. A. M. Corstjens, J. P. M. de Vrind and E. W. de Vrind-de Jong (2000). "Bacterial Mn2+ oxidizing systems and multicopper oxidases: An overview of mechanisms and functions." Geomicrobiology Journal 17: 1-24.

Caspi, R., B. M. Tebo and M. G. Haygood (1998). "c-type cytochromes and manganese oxidation in Pseudomonas putida strain MnB1." Applied and Environmental Microbiology 64: 3549-3555.

de Vrind, J. P., G. J. Brouwers, P. L. A. M. Corstjens, J. Den Dulk and E. W. De Vrind-De Jong (1998). "The cytochrome c maturation operon is involved in manganese oxidation in Pseudomonas putida GB-1." Applied and Environmental Microbiology 64: 3556-3562.

Francis, C. A. and B. M. Tebo (2001). "Diversity of cumA multicopper oxidase genes from Mn(II)-oxidizing and non-oxidizing Pseudomonas strains." Applied and Environmental Microbiology 67: 4272-4278.

McCarthy, J. K., R. Verity, B. Clement and B. M. Tebo (in preparation). "Identification of a second multicopper oxidase gene, mnxG, involved in Mn(II) oxidation in Pseudomonas putida Strain GB-1."

Ngo, T. V., B. M. Tebo and J. K. McCarthy (in preparation). "Regulation of Mn(II) oxidation, alginate biosynthesis and pyoverdine transport by a sigma factor repressor in Pseudomonas putida strain GB-1." in preparation.

Okazaki, M., T. Sugita, M. Shimizu, Y. Ohode, K. Iwamoto, E. W. de Vrind-de Jong, J. P. M. de Vrind and P. L. A. M. Corstjens (1997). "Partial purification and characterization of manganese-oxidizing factors of Pseudomonas fluorescens GB-1." Applied and Environmental Microbiology 63: 4793-4799.

Parker, D. L., G. Sposito and B. M. Tebo (2004). "Manganese(III) binding to a pyoverdine siderophore produced by a manganese(II)-oxidizing bacterium." Geochimica et Cosmochimica Acta 68(23): 4809-4820.

Tebo, B. M., J. R. Bargar, B. Clement, G. Dick, K. J. Murray, D. L. Parker, R. Verity and S. M. Webb (2004). "Biogenic Manganese Oxides: Properties and Mechanisms of Formation." Annual Reviews of Earth and Planetary Science 32: 287-328.