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Prochlorococcus sp. WH8102
   
   
 


Photo: John Waterbury,
Woods Hole Oceanographic Institute

Marine unicellular cyanobacteria of the synechococcus group occupy an important position at the base of the marine food web: they are abundant in the world's oceans and as a result are major primary producers on a global scale and one of the most numerous genomes on earth (1). Members of this group are adapted to life in the ocean; they are obligately marine, having elevated growth requirements not only for Na+, but also for Cl-, Mg2+, and Ca2+; they have the ability to acquire major nutrients and trace metals at the submicromolar concentrations found in the oligotrophic open seas(1, 2), and their light-harvesting apparatus is uniquely adapted to the spectral quality of light in the ocean (3). Furthermore, a third of the open ocean isolates of synechococcus possess a unique type of swimming motility not seen in any other type of microorganism: they propel themselves through seawater at speeds of up to 25 mm/sec in the absence of any demonstrable external organelle (4). They do not use their motility to respond to light gradients, but instead to respond to extremely small gradients (10-9 to 10-10 M) of nitrogenous compounds (5). Members of the marine synechococccus group are closely related at the level of 16s rRNA , and the motile strains form a monophyletic cluster within this group (6).

Synechococcus sp. strain WH8102 is a motile strain that can be grown in both natural and artificial seawater liquid media as well as on plates and is amenable to biochemical and genetic manipulation (7, 8, 9). The availability of the complete sequence of the genome of synechococcus WH8102 will provide insights not only into the unique adaptations of this cyanobacterial group to the marine environment, including mechanisms of nutrient (10, 11) and metal transport, chemotaxis, motility, and viral interactions,but also into what factors might be ultimately important in controlling primary productivity in the oceans. Furthermore, marine synechococcus spp. coexist with the other abundant unicellular marine cyanobacterial group, prochlorococcus. A major difference between the synechococcus and prochlorococcus groups lies in their light-harvesting apparatus, with synechococcus utilizing chlorophyll a, and prochlorococcus relying on divinyl chlorophylls a and b. A comparative analysis of their genomes should allow insights not only into the evolution of light-harvesting complexes, but also into cyanobacterial diversification in the oceans, including adaptations to different marine niches (12).

References

  1. Waterbury, J. B., S. W. Watson, F. W. Valois, and D. G. Franks. 1986. Biological and ecological characterization of the marine unicellular cyanobacterium synechococcus. Can. Bull. Fish. Aquat. Sci. 214:71-120.
  2. Carr, N.G., and N. H. Mann. 1994. The oceanic cyanobacterial picoplankton, p. 27-48. In D. A. Bryant (ed.), the Molecular biology of cyanobacteria. Kluwer Academic publishers, Boston.
  3. Wood, A. M. 1985. Adapatation of the photosynthetic apparatus of marine ultraphytoplankton to natural light fields. Nature. 316:253-255.
  4. Waterbury, J. B., J. M. Willey, D. G. Franks, F. W. Valois, and S. W. Watson. 1985. A cyanobacterium capable of swimming motility. Science. 230:74-76.
  5. Willey, J. M. and J. B. Waterbury. 1989. Chemotaxis toward nitrogenous compounds by swimming strains of marine synechococcus spp. Appl. Environ. Microbiol. 55:1888-1894.
  6. Toledo, G., B. Palenik, and B. Brahamsha. 1999. Swimming marine synechococcus strains with widely different photosynthetic pigment ratios from a monophyletic group. Appl. Environ. Microbiol. 65:5247-5251.
  7. Waterbury, J. B. and J. M. Willey. 1988. Isolation and growth of marine planktonic cyanobacteria. Methods Enzymol, 167:100-105.
  8. Brahamsha, B. 1996. A genetic manipulation system for oceanic cyanobacteria of the genus synechococcus. Appl. Environ. Microbiol. 62:1747-1751.
  9. Brahamsha, B. 1996. An abundant cell-surface polypeptide is required for swimming by the nonflagellated marine cyanobacterium synechococcus. Proc. Natl. Acad. Sci. USA 93:6504-6509.
  10. Scanlan, D. J., N. H. Mann, and N. G. Carr. 1993. The response of the picoplankton marine cyanobacterium synechococcus species WH7803 to phosphate starvation involves a protein homologous to the periplasmic phosphate-binding protein of escherichia coli. Mol. Microbiol. 10:181-191.
  11. Lindell, D., E. Padan and A. F. Post. 1998. Regulation of ntcA expression and nitrite uptake in the marine synechococcus sp. strain WH 7803. J. Bacteriol.,180:1878-1886.
  12. Ferris, M. J. and B. Palenik. 1998. Niche adaptation in ocean cyanobacteria. Nature. 396:226-228.