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This
draft was generated using a whole genome shotgun method. The genomic DNA
was purified from a homokaryotic strain RP-78 and five principal small
insert (3-4 Kbp) pUC18 plasmid libraries were generated. Total 619,903
end sequences approximately 10.5X sequence coverage were produced. To
further corroborate the long-range structure of the assembled genome,
we end-sequenced 2000 clones from a cosmid library (1.5X clone coverage),
and confirmed that the vast majority of end-sequences were found in opposing
orientations within three standard deviations of each other. |
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Catholic University,
Departmento de Genética Molecular y Microbiologíca,
Facultad de Ciencias Biológicas, Pontifica Universidad Católica
de Chile, Santiago, Chile and Millenium Institute for Fundamental
and Applied (Santiago, Chile)
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Department
of Physics, University of California, Berkeley (Berkeley, CA,
USA)
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Novozyme Biotech,
Inc. (Davis, CA, USA)
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Oak Ridge National
Laboratory (Oak Ridge, TN, USA)
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Center National
De La Recherche Scientifique (France)
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USDA, Forest
Service, Forest Products Laboratory (Madison, WI, USA)
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Department of
Biological Sciences, Columbia University (New York, NA USA)
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This
work was performed under the auspices of the US Department of Energy's
Office of Science, Biological and Environmental Research Program and the
by the University of California, Lawrence Livermore National Laboratory
under Contract No. W-7405-Eng-48, Lawrence Berkeley National Laboratory
under contract No. DE-AC03-76SF00098 and Los Alamos National Laboratory
under contract No. W-7405-ENG-36. |